Signal-regulatory protein gamma (SIRPG), partial, human, recombinant

Organism: Homo sapiens (Human). Source: Yeast. Expression Region: 29-360aa. Protein Length: Extracellular Domain. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: EEELQMIQPE KLLLVTVGKT ATLHCTVTSL LPVGPVLWFR GVGPGRELIY NQKEGHFPRV TTVSDLTKRN NMDFSIRISS ITPADVGTYY CVKFRKGSPE NVEFKSGPGT EMALGAKPSA PVVLGPAART TPEHTVSFTC ESHGFSPRDI TLKWFKNGNE LSDFQTNVDP TGQSVAYSIR STARVVLDPW DVRSQVICEV AHVTLQGDPL RGTANLSEAI RVPPTLEVTQ QPMRVGNQVN VTCQVRKFYP QSLQLTWSEN GNVCQRETAS TLTENKDGTY NWTSWFLVNI SDQRDDVVLT CQVKHDGQLA VSKRLALEVT VHQKDQSSDA TP. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Probable immunoglobulin-like cell surface receptor. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation. Reference: "Adhesion of human T cells to antigen-presenting cells through SIRPbeta2-CD47 interaction costimulates T-cell proliferation."Piccio L., Vermi W., Boles K.S., Fuchs A., Strader C.A., Facchetti F., Cella M., Colonna M.Blood 105:2421-2427(2005). Function: Probable immunoglobulin-like cell surface receptor. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation.