Serine/threonine-protein kinase 16 (STK16), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-305aa. Protein Length: Full Length. Tag Info: N-terminal GST-tagged. Target Protein Sequence: GHALCVCSRG TVIIDNKRYL FIQKLGEGGF SYVDLVEGLH DGHFYALKRI LCHEQQDREE AQREADMHRL FNHPNILRLV AYCLRERGAK HEAWLLLPFF KRGTLWNEIE RLKDKGNFLT EDQILWLLLG ICRGLEAIHA KGYAHRDLKP TNILLGDEGQ PVLMDLGSMN QACIHVEGSR QALTLQDWAA QRCTISYRAP ELFSVQSHCV IDERTDVWSL GCVLYAMMFG EGPYDMVFQK GDSVALAVQN QLSIPQSPRH SSALRQLLNS MMTVDPHQRP HIPLLLSQLE ALQPPAPGQH TTQI. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Membrane-associated protein kinase that phosphorylates on serine and threonine residues. In vitro substrates include DRG1, ENO1 and EIF4EBP1. Also autophosphorylates. May be involved in secretory vesicle trafficking or intracellular signaling. May have a role in regulating stromal-epithelial interactions that occur during ductal morphogenesis in the mammary gland. May be involved in TGF-beta signaling. Able to autophosphorylate on Tyr residue, it is however unclear whether it has tyrosine-protein kinase toward other proteins. Reference: "A novel transcriptional factor with Ser/Thr kinase activity involved in the transforming growth factor (TGF)-beta signalling pathway." Ohta S., Takeuchi M., Deguchi M., Tsuji T., Gahara Y., Nagata K. Biochem. J. 350:395-404(2000). Function: Membrane-associated protein kinase that phosphorylates on serine and threonine residues. In vitro substrates include DRG1, ENO1 and EIF4EBP1. Also autophosphorylates. May be involved in secretory vesicle trafficking or intracellular signaling. May have a role in regulating stromal-epithelial interactions that occur during ductal morphogenesis in the mammary gland. May be involved in TGF-beta signaling. Able to autophosphorylate on Tyr residue, it is however unclear whether it has tyrosine-protein kinase toward other proteins.