Semaphorin-4D (Sema4d), partial (Active), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Mammalian cell. Expression Region: 24-733aa. Protein Length: Partial. Tag Info: C-terminal mFc-tagged. Target Protein Sequence: FAPVPRLTWE HGEVGLVQFH KPGIFNYSAL LMSEDKDTLY VGAREAVFAV NALNISEKQH EVYWKVSEDK KSKCAEKGKS KQTECLNYIR VLQPLSSTSL YVCGTNAFQP TCDHLNLTSF KFLGKSEDGK GRCPFDPAHS YTSVMVGGEL YSGTSYNFLG SEPIISRNSS HSPLRTEYAI PWLNEPSFVF ADVIQKSPDG PEGEDDKVYF FFTEVSVEYE FVFKLMIPRV ARVCKGDQGG LRTLQKKWTS FLKARLICSK PDSGLVFNIL QDVFVLRAPG LKEPVFYAVF TPQLNNVGLS AVCAYTLATV EAVFSRGKYM QSATVEQSHT KWVRYNGPVP TPRPGACIDS EARAANYTSS LNLPDKTLQF VKDHPLMDDS VTPIDNRPKL IKKDVNYTQI VVDRTQALDG TFYDVMFIST DRGALHKAVI LTKEVHVIEE TQLFRDSEPV LTLLLSSKKG RKFVYAGSNS GVVQAPLAFC EKHGSCEDCV LARDPYCAWS PAIKACVTLH QEEASSRGWI QDMSGDTSSC LDKSKESFNQ HFFKHGGTAE LKCFQKSNLA RVVWKFQNGE LKAASPKYGF VGRKHLLIFN LSDGDSGVYQ CLSEERVRNK TVSQLLAKHV LEVKMVPRTP PSPTSEDAQT EGSKITSKMP VASTQGSSPP TPALWATSPR AATLPPKSSS GTSCEPKMVI NTVPQLHSEK TVYLKSSDNR. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Pepinemab at 2 µg/ml can bind Mouse Sema4d, the EC50 is 4.878-7.446 ng/ml. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered PBS, 6% Trehalose, pH 7.4. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: n/a. Reference: n/a. Function: nan