Semaphorin-3A (Sema3a), rat, recombinant

Organism: Rattus norvegicus (Rat). Source: E.coli. Expression Region: 21-772aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: NYANGKNNVP RLKLSYKEML ESNNVITFNG LANSSSYHTF LLDEERSRLY VGAKDHIFSF NLVNIKDFQK IVWPVSYTRR DECKWAGKDI LKECANFIKV LKAYNQTHLY ACGTGAFHPI CTYIEVGHHP EDNIFKLQDS HFENGRGKSP YDPKLLTASL LIDGELYSGT AADFMGRDFA IFRTLGHHHP IRTEQHDSRW LNDPRFISAH LIPESDNPED DKVYFFFREN AIDGEHSGKA THARIGQICK NDFGGHRSLV NKWTTFLKAR LICSVPGPNG IDTHFDELQD VFLMNSKDPK NPIVYGVFTT SSNIFKGSAV CMYSMSDVRR VFLGPYAHRD GPNYQWVPYQ GRVPYPRPGT CPSKTFGGFD STKDLPDDVI TFARSHPAMY NPVFPINNRP IMIKTDVNYQ FTQIVVDRVD AEDGQYDVMF IGTDVGTVLK VVSVPKETWH DLEEVLLEEM TVFREPTTIS AMELSTKQQQ LYIGSTAGVA QLPLHRCDIY GKACAECCLA RDPYCAWDGS SCSRYFPTAK RRTRRQDIRN GDPLTHCSDL QHHDNHHGHS LEERIIYGVE NSSTFLECSP KSQRALVYWQ FQRRNEDRKE EIRVGDHIIR TEQGLLLRSL QKKDSGNYLC HAVEHGFMQT LLKVTLEVID TEHLEELLHK DDDGDGSKTK EMSSSMTPSQ KVWYRDFMQL INHPNLNTMD EFCEQVWKRD RKQRRQRPGH SQGSSNKWKH MQESKKGRNR RTHEFERAPR SV. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May be involved in guiding growing axons towards their targets by forming a molecular boundary that instructs axons to engage in the formation of specific nerve tracts. Binds to neuropilin. Involved in the development of the olfactory system and in neuronal control of puberty. Reference: "Anatomy of rat semaphorin III/collapsin-1 mRNA expression and relationship to developing nerve tracts during neuroembryogenesis."Giger R.J., Wolfer D.P., De Wit G.M.J., Verhaagen J.J. Comp. Neurol. 375:378-392(1996). Function: May be involved in guiding growing axons towards their targets by forming a molecular boundary that instructs axons to engage in the formation of specific nerve tracts. Binds to neuropilin. Involved in the development of the olfactory system and in neuronal control of puberty (By similarity).