Resistin, Recombinant, Human (FIZZ3, Found in Inflammatory Zone 3, ADSF, Adipose Tissue-Specific Sec

The adipocytokine resistin which belongs to a family of cysteine-rich C-terminal proteins known as resistin-like molecules (RELM, RELM-alpha/FIZZ 1 and RELM-beta/FIZZ 2) of FIZZ (found in inflammatory zone) are thought to be involved in inflammatory processes. Previous studies in mice showed that resistin impairs glucose tolerance and insulin action. In addition, resistin also inhibits adipogenesis in murine 3T3-L1 cells. Therefore resistin has also been proposed as an adipocyte-secreted factor thought to link obesity and T2DM. Sequence: Human resistin (aa19-108) is fused at the N-terminus to a FLAG(R)-tag, expressed in HEK 293 cells. Biological Activity: Inhibits insulin-stimulated glucose uptake in L6 skeletal muscle cells. Molecular Weight: ~13kD (SDS-PAGE), Endotoxin: <0.01EU/ug (LAL test, Lonza), Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.