Raf1, Recombinant, Human

Human RAF1 (GenBank Accession No. NM_002880), aa306-648, containing the mutations Y340D and Y341D, expressed in an Baculovirus infected Sf9 cell expression system. Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Specific Activity: As reported. Typical value:~160 pmol/min/ug. Assay conditions: 10uM ATP, 1uM MEK1 (K97R), 1hour, Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.