Proteinase K Solution 20mg/ml

Proteinase K is classified as a non-specific serine protease. It is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. Applications:  Isolation of high molecular weight DNA ,  Isolation of plasmid and genomic DNA ,  Isolation of RNA ,  Inactivation of RNase and DNase activities , Advantages:  Ready to use, provided in liquid form containing 40% glycerol.,  Smaller contamination risk with liquid form.,  Can be stored at -20°C still having the enzyme in liquid form.,  Excellent stability, Notes: Proteinase K is not inactivated by metal-chelating agents like EDTA, by thiol-reactive reagents (such as monoiodoacetic acid, p-chloromercuribenzoate) or by specific trypsin and chymotrypsin inhibitors. Phenylmethylsulfonyl fluoride and diisopropyl phosphorofluoridate completely inhibit the enzyme. , Proteinase K is stable over a wide pH range: 4-12.5. , Raising the temperature of the reaction from 37°C to 50°-65°C can increase the activity of Proteinase K., Proteinase K can be inactivated at 95°C for 10 minutes (some enzymatic activity may still remain), Proteinase K retains its activity in the presence of SDS (0.5-1%) or urea (1-4M)., Depending on the application the enzyme is used in the absence or presence of 0.2-1% SDS or 1-4M urea. Nomenclature: EC=3.4.21.64, PROK, Endopeptidase K, Tritirachium Alkaline Proteinase, Source: Tritirachium album limber, Concentration: ~20mg/ml, Form: Supplied as a liquid in 10mM Tris HCl, pH 7.5, 1mM calcium acetate, 40% glycerol. Recommended Dilutions: The recommended working concentration for Proteinase K is 0.05-1mg/ml. Use 3ul of a 20mg/ml solution per 1.5ml of bacterial culture. Optimal dilutions to be determined by researcher. Solubility: Colorless to pale yellow, clear, complete, pH: 7.5 ± 0.2, Specific Activity: >30 units/mg, Unit Definition: One unit liberates 1umole of Folin positive amino acids, measured as Tyrosine, at 37°C, pH 7.5, using urea-denatured hemoglobin as the substrate. Quality Control: Nicking Activity: None Detected, RNases: None Detected, Exonucleases: None Detected, Phosphatases: None Detected, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.