Protein-glutamine gamma-glutamyltransferase 4 (TGM4), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-684aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: MMDASKELQV LHIDFLNQDN AVSHHTWEFQ TSSPVFRRGQ VFHLRLVLNQ PLQSYHQLKL EFSTGPNPSI AKHTLVVLDP RTPSDHYNWQ ATLQNESGKE VTVAVTSSPN AILGKYQLNV KTGNHILKSE ENILYLLFNP WCKEDMVFMP DEDERKEYIL NDTGCHYVGA ARSIKCKPWN FGQFEKNVLD CCISLLTESS LKPTDRRDPV LVCRAMCAMM SFEKGQGVLI GNWTGDYEGG TAPYKWTGSA PILQQYYNTK QAVCFGQCWV FAGILTTVLR ALGIPARSVT GFDSAHDTER NLTVDTYVNE NGEKITSMTH DSVWNFHVWT DAWMKRPDLP KGYDGWQAVD ATPQERSQGV FCCGPSPLTA IRKGDIFIVY DTRFVFSEVN GDRLIWLVKM VNGQEELHVI SMETTSIGKN ISTKAVGQDR RRDITYEYKY PEGSSEERQV MDHAFLLLSS EREHRRPVKE NFLHMSVQSD DVLLGNSVNF TVILKRKTAA LQNVNILGSF ELQLYTGKKM AKLCDLNKTS QIQGQVSEVT LTLDSKTYIN SLAILDDEPV IRGFIIAEIV ESKEIMASEV FTSFQYPEFS IELPNTGRIG QLLVCNCIFK NTLAIPLTDV KFSLESLGIS SLQTSDHGTV QPGETIQSQI KCTPIKTGPK KFIVKLSSKQ VKEINAQKIV LITK. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Associated with the mammalian reproductive process. Catalyzes the cross-linking of proteins and the conjugation of polyamines to specific proteins in the seminal tract. Reference: "The prostate transglutaminase (TGase-4, TGaseP) regulates the interaction of prostate cancer and vascular endothelial cells, a potential role for the ROCK pathway." Jiang W.G., Ablin R.J., Kynaston H.G., Mason M.D. Microvasc Res 77:150-157(2009). Function: nan