Pregnancy-associated glycoprotein 2 (PAG2), bovine, recombinant

Organism: Bos taurus (Bovine). Source: Yeast. Expression Region: 22-376aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: KKMKTLRETL REKNLLNNFL EEQAYRLSKN DSKITIHPLR NYLDTAYVGN ITIGTPPQEF RVVFDTGSAN LWVPCITCTS PACYTHKTFN PQNSSSFREV GSPITIFYGS GIIQGFLGSD TVRIGNLVSP EQSFGLSLEE YGFDSLPFDG ILGLAFPAMG IEDTIPIFDN LWSHGAFSEP VFAFYLNTNK PEGSVVMFGG VDHRYYKGEL NWIPVSQTSH WQISMNNISM NGTVTACSCG CEALLDTGTS MIYGPTKLVT NIHKLMNARL ENSEYVVSCD AVKTLPPVIF NINGIDYPLR PQAYIIKIQN SCRSVFQGGT ENSSLNTWIL GDIFLRQYFS VFDRKNRRIG LAPAV. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: PAG2 or a processed derivative of this molecule might represent a factor that binds the LH receptor. Reference: "The glycosylation of pregnancy-associated glycoproteins and prolactin-related protein-I in bovine binucleate trophoblast giant cells changes before parturition." Klisch K., Boos A., Friedrich M., Herzog K., Feldmann M., Sousa N., Beckers J., Leiser R., Schuler G. Reproduction 132:791-798(2006). Function: nan