Poly [ADP-ribose] polymerase tankyrase-2 (TNKS2), partial, recombinant human

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 873-1161aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: GVDFSITQFV RNLGLEHLMD IFEREQITLD VLVEMGHKEL KEIGINAYGH RHKLIKGVER LISGQQGLNP YLTLNTSGSG TILIDLSPDD KEFQSVEEEM QSTVREHRDG GHAGGIFNRY NILKIQKVCN KKLWERYTHR RKEVSEENHN HANERMLFHG SPFVNAIIHK GFDERHAYIG GMFGAGIYFA ENSSKSNQYV YGIGGGTGCP VHKDRSCYIC HRQLLFCRVT LGKSFLQFSA MKMAHSPPGH HSVTGRPSVN GLALAEYVIY RGEQAYPEYL ITYQIMRPE. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates poly-ADP-ribosylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination. Mediates poly-ADP-ribosylation of TERF1, thereby contributing to the regulation of telomere length. Stimulates 26S proteasome activity. Reference: n/a. Function: nan