Phosphodiesterase PDE1B, Recombinant, Human

Human PDE1B (GenBank Accession No. NM_000924), full length with N-terminal GST tag. Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity, profiling. Specific Activity: ~400U/ug. One unit is defined as the amount of enzyme that will convert 1pmole of 3', 5'-cAMP to 5' AMP per min at 30°C in a reaction buffer containing 10mM Tris-HCl, pH 7.4, 0.2mM MgCl2. Assay buffer: 10mM tRIS-HCl, pH 7.4, 0.2mM MgCl2, 0.02mM CaCl2, 240nM Calmodulin, 3'5'-cAMP substrtae: 0.4mM, PDE1B: 2ng/ul. Storage and Stability: Aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.