Peptidylglycine alpha-amidating monooxygenase (Pam), partial, recombinant rat

Organism: Rattus norvegicus (Rat). Source: Mammalian cell. Expression Region: 36-820aa. Protein Length: Partial. Tag Info: N-terminal 8xHis-tagged. Target Protein Sequence: FKETTRSFSN ECLGTIGPVT PLDASDFALD IRMPGVTPKE SDTYFCMSMR LPVDEEAFVI DFKPRASMDT VHHMLLFGCN MPSSTGSYWF CDEGTCTDKA NILYAWARNA PPTRLPKGVG FRVGGETGSK YFVLQVHYGD ISAFRDNHKD CSGVSVHLTR VPQPLIAGMY LMMSVDTVIP PGEKVVNADI SCQYKMYPMH VFAYRVHTHH LGKVVSGYRV RNGQWTLIGR QNPQLPQAFY PVEHPVDVTF GDILAARCVF TGEGRTEATH IGGTSSDEMC NLYIMYYMEA KYALSFMTCT KNVAPDMFRT IPAEANIPIP VKPDMVMMHG HHKEAENKEK SALMQQPKQG EEEVLEQGDF YSLLSKLLGE REDVHVHKYN PTEKTESGSD LVAEIANVVQ KKDLGRSDAR EGAEHEEWGN AILVRDRIHR FHQLESTLRP AESRAFSFQQ PGEGPWEPEP SGDFHVEEEL DWPGVYLLPG QVSGVALDSK NNLVIFHRGD HVWDGNSFDS KFVYQQRGLG PIEEDTILVI DPNNAEILQS SGKNLFYLPH GLSIDTDGNY WVTDVALHQV FKLDPHSKEG PLLILGRSMQ PGSDQNHFCQ PTDVAVEPST GAVFVSDGYC NSRIVQFSPS GKFVTQWGEE SSGSSPRPGQ FSVPHSLALV PHLDQLCVAD RENGRIQCFK TDTKEFVREI KHASFGRNVF AISYIPGFLF AVNGKPYFGD QEPVQGFVMN FSSGEIIDVF KPVRKHFDMP HDIVASEDGT VYIGDAHTNT VWKFTLTEKM EHRSV. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Bifunctional enzyme that catalyzes the post-translational modification of inactive peptidylglycine precursors to the corresponding bioactive alpha-amidated peptides, a terminal modification in biosynthesis of many neural and endocrine peptides. Reference: n/a. Function: nan