Methionine aminopeptidase 2 (METAP2), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-478aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: AGVEEVAASG SHLNGDLDPD DREEGAASTA EEAAKKKRRK KKKSKGPSAA GEQEPDKESG ASVDEVARQL ERSALEDKER DEDDEDGDGD GDGATGKKKK KKKKKRGPKV QTDPPSVPIC DLYPNGVFPK GQECEYPPTQ DGRTAAWRTT SEEKKALDQA SEEIWNDFRE AAEAHRQVRK YVMSWIKPGM TMIEICEKLE DCSRKLIKEN GLNAGLAFPT GCSLNNCAAH YTPNAGDTTV LQYDDICKID FGTHISGRII DCAFTVTFNP KYDTLLKAVK DATNTGIKCA GIDVRLCDVG EAIQEVMESY EVEIDGKTYQ VKPIRNLNGH SIGQYRIHAG KTVPIVKGGE ATRMEEGEVY AIETFGSTGK GVVHDDMECS HYMKNFDVGH VPIRLPRTKH LLNVINENFG TLAFCRRWLD RLGESKYLMA LKNLCDLGIV DPYPPLCDIK GSYTAQFEHT ILLRPTCKEV VSRGDDY. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged. The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis. Reference: "Toward a comprehensive characterization of a human cancer cell phosphoproteome." Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., Mohammed S. J. Proteome Res. 12:260-271(2013). Function: Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo., FUNCTION