MerTK, Fc Chimera, Recombinant, Human (Mer, Receptor Tyrosine Protein Kinase Mer, c-Eyk, c-mer proto

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Recent studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions. Sequence Information: Human Mer, Arg26-Ala499 (Accession #Q12866) - IEGRMD - Human IgG1 (Pro100-Lys330), Predicted Molecular Weight: ~78.9kD (monomer), Observed Molecular Weight: 100-110kD (SDS-PAGE, reducing conditions), Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized rhMer/Fc Chimera at 10ug/ml (100ul/well) can bind rhGas6 with a linear range of 0.03-1ug/ml. Endotoxin: ~0.1EU/1ug (LAL), Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.