Melanotransferrin (Mfi2), partial, rabbit, recombinant

Organism: Oryctolagus cuniculus (Rabbit). Source: E.coli. Expression Region: 20-710aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: SVEVRWCTAS EPEQQKCEDM SQAFREAGLQ PALLCVQGTS ADHCVQLIAA HEADAITLDG GAIYEAGKEH GLKPVVGEVY DQEVGTSYYA VAVVKRSSNV TINTLRGVKS CHTGINRTVG WNVPVGYLVD SGRLSVMGCD VLKAVSEYFG GSCVPGAGET RYSESLCRLC RGDTSGEGVC DKSPLERYYD YSGAFRCLAE GAGDVAFVKH STVLENTDGR TLPSWGHMLM SRDFELLCRD GSRASVTEWQ HCHLARVPAH AVVVRADTDA GLIFRLLNEG QRLFSHEGSS FQMFSSEAYG QKNLLFKDST LELVPIATQT YEAWLGPEYL HAMKGLLCDP NRLPPYLRWC VLSTPEIQKC GDMAVAFSRQ RLKPEIQCVS AESPQHCMEQ IQAGHIDAVT LNGEDIHTAG KTYGLIPAAG ELYAADDRSN SYFVVAVVKR DSAYAFTVDE LRGKRSCHPG FGSPAGWDVP VGALIHWGYI RPRNCDVLTA VGQFFNASCV PVNNPKKYPS SLCALCVGDE QGRNKCTGNS QERYYGDSGA FRCLVEGAGD VAFVKHTTIF DNTNGHNPEP WAAHLRSQDY ELLCPNGARA EAHQFAACNL AQIPSHAVMV RPDTNIFTVY GLLDKAQDLF GDDHNKNGFK MFDSSSYHGR DLLFKDATVR AVPVGERTTY QDWLGPDYVA ALEGMQSQRC S. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Involved in iron cellular uptake. Ses to be internalized and then recycled back to the cell mbrane. Binds a single atom of iron per subunit. Could also bind zinc . Reference: Expression of membrane-bound transferrin-like protein p97 on the cell surface of chondrocyte.Kawamoto T., Pan H., Yan W., Ishida H., Usui E., Oda R., Nakamasu K., Noshiro M., Kawashima-Ohya Y., Fujii M., Shintani H., Okada Y., Kato Y.Eur. J. Biochem. 256:503-509(1998). Function: Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc (By similarity).