Maltase-glucoamylase (MGAM), partial, recombinant human

Organism: Homo sapiens (Human). Source: Baculovirus. Expression Region: 960-1853aa. Protein Length: Partial of Isoform 1. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: DEEKIDCYPD ENGASAENCT ARGCIWEASN SSGVPFCYFV NDLYSVSDVQ YNSHGATADI SLKSSVYANA FPSTPVNPLR LDVTYHKNEM LQFKIYDPNK NRYEVPVPLN IPSMPSSTPE GQLYDVLIKK NPFGIEIRRK STGTIIWDSQ LLGFTFSDMF IRISTRLPSK YLYGFGETEH RSYRRDLEWH TWGMFSRDQP PGYKKNSYGV HPYYMGLEED GSAHGVLLLN SNAMDVTFQP LPALTYRTTG GVLDFYVFLG PTPELVTQQY TELIGRPVMV PYWSLGFQLC RYGYQNDSEI ASLYDEMVAA QIPYDVQYSD IDYMERQLDF TLSPKFAGFP ALINRMKADG MRVILILDPA ISGNETQPYP AFTRGVEDDV FIKYPNDGDI VWGKVWPDFP DVVVNGSLDW DSQVELYRAY VAFPDFFRNS TAKWWKREIE ELYNNPQNPE RSLKFDGMWI DMNEPSSFVN GAVSPGCRDA SLNHPPYMPH LESRDRGLSS KTLCMESQQI LPDGSLVQHY NVHNLYGWSQ TRPTYEAVQE VTGQRGVVIT RSTFPSSGRW AGHWLGDNTA AWDQLKKSII GMMEFSLFGI SYTGADICGF FQDAEYEMCV RWMQLGAFYP FSRNHNTIGT RRQDPVSWDV AFVNISRTVL QTRYTLLPYL YTLMHKAHTE GVTVVRPLLH EFVSDQVTWD IDSQFLLGPA FLVSPVLERN ARNVTAYFPR ARWYDYYTGV DINARGEWKT LPAPLDHINL HVRGGYILPW QEPALNTHLS RQKFMGFKIA LDDEGTAGGW LFWDDGQSID TYGKGLYYLA SFSASQNTMQ SHIIFNNYIT GTNPLKLGYI EIWGVGSVPV TSVSISVSGM VITPSFNNDP TTQVLSIDVT DRNISLHNFT SLTW. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Alpha-(1,4) exo-glucosidase involved in breakdown of dietary starch oligosaccharides in small intestine. Cleaves the non-reducing alpha-(1,4)-linked glucose residue in linear dextrins with retention of anomeric center stereochemistry. Mainly hydrolyzes short length oligomaltoses having two to seven glucose residues. Can cleave alpha-(1,2), alpha-(1,3) and alpha-(1,6) glycosidic linkages with lower efficiency, whereas beta glycosidic linkages are usually not hydrolyzed. Reference: "Contribution of the Individual Small Intestinal alpha-Glucosidases to Digestion of Unusual alpha-Linked Glycemic Disaccharides." Lee B.H., Rose D.R., Lin A.H., Quezada-Calvillo R., Nichols B.L., Hamaker B.R. J. Agric. Food Chem. 64:6487-6494(2016). Function: nan