INSR, Insulin receptor, active human recombinant protein

Active human receptor (INSR) (GenBank Accession No. NM_001079817) (amino acids 999-end) with N-terminal GST tag, MW=67 kDa. Expressed in a baculovirus expression system. Assay condition: The enzyme reaction was carried out for 1h at room temperature in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EDTA, 0.01% BRIJ- 35 and 200 BM of ATP. Substrate : 4 BM Tyr peptide 1 from Invitrogen. Application: Enzyme kinetics, autophosphorylation, and phosphorylation of target substrates.