Fusion glycoprotein F0 (F), partial, human metapneumovirus, recombinant

Organism: Human metapneumovirus (strain CAN97-83) (HMPV). Source: Baculovirus. Expression Region: 20-102aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: KESYLEESCS TITEGYLSVL RTGWYTNVFT LEVGDVENLT CSDGPSLIKT ELDLTKSALR ELKTVSADQL AREEQIENPR QSR. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Inactive precursor that is cleaved to give rise to the mature F1 and F2 fusion glycoproteins. Reference: "Human metapneumovirus (HMPV) binding and infection are mediated by interactions between the HMPV fusion protein and heparan sulfate." Chang A., Masante C., Buchholz U.J., Dutch R.E. J. Virol. 86:3230-3243(2012). Function: nan