Flagellin (CBO2730), Clostridium botulinum, recombinant

Organism: Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A). Source: E.coli. Expression Region: 1-275aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: MIINHNLNAM NAHRQMAINT GNNGKAIEKL SSGLRINRAG DDAAGLAISE KMRGQIRGLN QASRNSQDAI SLIQTAEGAL NETHSILQRM RELTVQAAND TNVTVDRENL QKEVAELQSE INRISSQTQF NTKSLINGAV SKDADAMTFQ IGANSGQTIK LTIGSMDAKT LNVDKIKLDD TAANVTKQIA TIDNAINSVS KERAKLGANQ NRLEHTIANL DNSAENLQAA ESRIRDVDMA KEMMNFTKTN ILTQAAQAML AQANQAPQGV LQLLR. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. Reference: "Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes." Sebaihia M., Peck M.W., Minton N.P., Thomson N.R., Holden M.T.G., Mitchell W.J., Carter A.T., Bentley S.D., Mason D.R., Crossman L., Paul C.J., Ivens A., Wells-Bennik M.H.J., Davis I.J., Cerdeno-Tarraga A.M., Churcher C., Quail M.A., Chillingworth T. Parkhill J.Genome Res. 17:1082-1092(2007). Function: nan