Elongation factor Tu, mitochondrial (TUFM), human, recombinant

Organism: Homo sapiens (Human). Source: Yeast. Expression Region: 47-455aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: AVEAKKTYVR DKPHVNVGTI GHVDHGKTTL TAAITKILAE GGGAKFKKYE EIDNAPEERA RGITINAAHV EYSTAARHYA HTDCPGHADY VKNMITGTAP LDGCILVVAA NDGPMPQTRE HLLLARQIGV EHVVVYVNKA DAVQDSEMVE LVELEIRELL TEFGYKGEET PVIVGSALCA LEGRDPELGL KSVQKLLDAV DTYIPVPARD LEKPFLLPVE AVYSVPGRGT VVTGTLERGI LKKGDECELL GHSKNIRTVV TGIEMFHKSL ERAEAGDNLG ALVRGLKRED LRRGLVMVKP GSIKPHQKVE AQVYILSKEE GGRHKPFVSH FMPVMFSLTW DMACRIILPP EKELAMPGED LKFNLILRQP MILEKGQRFT LRDGNRTIGT GLVTNTLAMT EEEKNIKWG. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Reference: Cloning, sequence analysis and expression of mammalian mitochondrial protein synthesis elongation factor Tu.Woriax V.L., Burkhart W.A., Spremulli L.L.Biochim. Biophys. Acta 1264:347-356(1995). Function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.