Elongation factor 2 (EEF2), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-858aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: VNFTVDQIRA IMDKKANIRN MSVIAHVDHG KSTLTDSLVC KAGIIASARA GETRFTDTRK DEQERCITIK STAISLFYEL SENDLNFIKQ SKDGAGFLIN LIDSPGHVDF SSEVTAALRV TDGALVVVDC VSGVCVQTET VLRQAIAERI KPVLMMNKMD RALLELQLEP EELYQTFQRI VENVNVIIST YGEGESGPMG NIMIDPVLGT VGFGSGLHGW AFTLKQFAEM YVAKFAAKGE GQLGPAERAK KVEDMMKKLW GDRYFDPANG KFSKSATSPE GKKLPRTFCQ LILDPIFKVF DAIMNFKKEE TAKLIEKLDI KLDSEDKDKE GKPLLKAVMR RWLPAGDALL QMITIHLPSP VTAQKYRCEL LYEGPPDDEA AMGIKSCDPK GPLMMYISKM VPTSDKGRFY AFGRVFSGLV STGLKVRIMG PNYTPGKKED LYLKPIQRTI LMMGRYVEPI EDVPCGNIVG LVGVDQFLVK TGTITTFEHA HNMRVMKFSV SPVVRVAVEA KNPADLPKLV EGLKRLAKSD PMVQCIIEES GEHIIAGAGE LHLEICLKDL EEDHACIPIK KSDPVVSYRE TVSEESNVLC LSKSPNKHNR LYMKARPFPD GLAEDIDKGE VSARQELKQR ARYLAEKYEW DVAEARKIWC FGPDGTGPNI LTDITKGVQY LNEIKDSVVA GFQWATKEGA LCEENMRGVR FDVHDVTLHA DAIHRGGGQI IPTARRCLYA SVLTAQPRLM EPIYLVEIQC PEQVVGGIYG VLNRKRGHVF EESQVAGTPM FVVKAYLPVN ESFGFTADLR SNTGGQAFPQ CVFDHWQILP GDPFDNSSRP SQVVAETRKR KGLKEGIPAL DNFLDKL. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movent of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Reference: Construction of a plasmid containing the complete coding region of human elongation factor 2.Hanes J., Freudenstein J., Rapp G., Scheit K.H.Biol. Chem. Hoppe-Seyler 373:201-204(1992). Function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.