EGFR (T790M), active human recombinant protein

Active human EGFR T790M (GenBank Accession No. NM_005228), (a.a. 668-1210) with N-terminal GST tag, MW= 88 kDa, expressed in baculovirus expression system. Assay condition: The enzyme reaction was carried out for 1h at room temperature in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EDTA, 0.01% BRIJ- 35 and 200 µM of ATP. Substrate : 2 µM Tyr peptide 4 from Invitrogen. Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.