E3 ubiquitin-protein ligase ZFP91 (ZFP91), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 242-570aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-B2M-tagged. Target Protein Sequence: ETPKPRRKSG KVKEEKEKKE IKVEVEVEVK EEENEIREDE EPPRKRGRRR KDDKSPRLPK RRKKPPIQYV RCEMEGCGTV LAHPRYLQHH IKYQHLLKKK YVCPHPSCGR LFRLQKQLLR HAKHHTDQRD YICEYCARAF KSSHNLAVHR MIHTGEKPLQ CEICGFTCRQ KASLNWHMKK HDADSFYQFS CNICGKKFEK KDSVVAHKAK SHPEVLIAEA LAANAGALIT STDILGTNPE SLTQPSDGQG LPLLPEPLGN STSGECLLLE AEGMSKSYCS GTERVSLMAD GKIFVGSGSS GGTEGLVMNS DILGATTEVL IEDSDSAGP. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Atypical E3 ubiquitin-protein ligase that mediates 'Lys-63'-linked ubiquitination of MAP3K14/NIK, leading to stabilize and activate MAP3K14/NIK. It thereby acts as an activator of the non-canonical NF-kappa-B2/NFKB2 pathway. May also play an important role in cell proliferation and/or anti-apoptosis. Reference: "An atypical E3 ligase zinc finger protein 91 stabilizes and activates NF-kappaB-inducing kinase via Lys63-linked ubiquitination." Jin X., Jin H.R., Jung H.S., Lee S.J., Lee J.H., Lee J.J. J. Biol. Chem. 285:30539-30547(2010). Function: nan