Cation-independent mannose-6-phosphate receptor (IGF2R), partial, bovine, recombinant

Organism: Bos taurus (Bovine). Source: E.coli. Expression Region: 628-772aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: LSRTEGDNCT VFDSQAGFSF DLTPLTKKDA YKVETDKYEF HINVCGPVSV GACPPDSGAC QVSRSDRKSW NLGRSNAKLS YYDGMIQLTY RDGTPYNNEK RTPRATLITF LCDRDAGVGF PEYQEEDNST YNFRWYTSYA CPEEP. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Mediates the transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex. The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation by binding DPP4. Reference: "Specificity of binding of clathrin adapters to signals on the mannose-6-phosphate/insulin-like growth factor II receptor." Glickman J.N., Conibear E., Pearse B.M.F. EMBO J. 8:1041-1047(1989). Function: nan