Cartilage matrix protein (MATN1), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 23-496aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: SPGLAPQSRG HLCRTRPTDL VFVVDSSRSV RPVEFEKVKV FLSQVIESLD VGPNATRVGM VNYASTVKQE FSLRAHVSKA ALLQAVRRIQ PLSTGTMTGL AIQFAITKAF GDAEGGRSRS PDISKVVIVV TDGRPQDSVQ DVSARARASG VELFAIGVGS VDKATLRQIA SEPQDEHVDY VESYSVIEKL SRKFQEAFCV VSDLCATGDH DCEQVCISSP GSYTCACHEG FTLNSDGKTC NVCSGGGGSS ATDLVFLIDG SKSVRPENFE LVKKFISQIV DTLDVSDKLA QVGLVQYSSS VRQEFPLGRF HTKKDIKAAV RNMSYMEKGT MTGAALKYLI DNSFTVSSGA RPGAQKVGIV FTDGRSQDYI NDAAKKAKDL GFKMFAVGVG NAVEDELREI ASEPVAEHYF YTADFKTINQ IGKKLQKKIC VEEDPCACES LVKFQAKVEG LLQALTRKLE AVSKRLAILE NTVV. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Cartilage matrix protein is a major component of the extracellular matrix of non-articular cartilage. It binds to collagen. Reference: "Interactions between the cartilage oligomeric matrix protein and matrilins. Implications for matrix assembly and the pathogenesis of chondrodysplasias." Mann H.H., Oezbek S., Engel J., Paulsson M., Wagener R. J. Biol. Chem. 279:25294-25298(2004). Function: nan