Carbonic anhydrase 5A, mitochondrial (CA5A), human, recombinant

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 39-305aa. Protein Length: Full Length of Mature Protein. Tag Info: Tag-Free. Target Protein Sequence: CAWQTSNNTL HPLWTVPVSV PGGTRQSPIN IQWRDSVYDP QLKPLRVSYE AASCLYIWNT GYLFQVEFDD ATEASGISGG PLENHYRLKQ FHFHWGAVNE GGSEHTVDGH AYPAELHLVH WNSVKYQNYK EAVVGENGLA VIGVFLKLGA HHQTLQRLVD ILPEIKHKDA RAAMRPFDPS TLLPTCWDYW TYAGSLTTPP LTESVTWIIQ KEPVEVAPSQ LSAFRTLLFS ALGEEEKMMV NNYRPLQPLM NRKVWASFQA TNEGTRS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Mitochondrial carbonic anhydrase that catalyzes the reversible conversion of carbon dioxide to bicarbonate/HCO3 . Mitochondria are impermeable to HCO3, and thus this intramitochondrial carbonic anhydrase is pivotal in providing HCO3 for multiple mitochondrial enzymes that catalyze the formation of essential metabolites of intermediary metabolism in the urea and Krebs cycles . Reference: "Genomic organization of the human gene (CA5) and pseudogene for mitochondrial carbonic anhydrase V and their localization to chromosomes 16q and 16p." Nagao Y., Batanian J.R., Clemente M.F., Sly W.S. Genomics 28:477-484(1995). Function: nan