BRD4 bromodomains 1 and 2 (human, recombinant, aa 49-460)

Bromodomain-containing protein 4 (BRD4) is a member of the bromodomain and extra-terminal domain (BET) family. It is a ubiquitously expressed nuclear protein with roles in a variety of cellular processes, including regulation of gene transcription, cell cycle progression, and viral genome segregation. BRD4 is comprised of two N-terminal bromodomains (BD1 and BD2) that can bind to acetylated lysine residues in histones, serving to couple histone acetylation marks to the transcriptional regulation of target promoters, an extra-terminal (ET) domain that facilitates protein-protein interactions, and a C-terminal motif, and can be expressed as either a long or short isoform generated via alternative splicing. In addition to binding acetylated lysine residues on histones, BRD4 can bind to a variety of non-histone proteins and protein complexes, including positive transcription elongation factor b (P-TEFb) and the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) envelope (E) protein, a transmembrane protein involved in CoV virion assembly and pathogenesis of the related virus, SARS-CoV. In mice, knock-down of Brd4 is embryonic lethal. Chromosomal translocations leading to the fusion of BRD4 with the nuclear protein in testis (NUT) gene and resulting in expression of BRD4-NUT fusion proteins are associated with NUT midline carcinoma (NMC). Inhibition of BRD4-NUT binding to chromatin by the BET bromodomain inhibitor (+)-JQ1 (Cay-11187) induces tumor regression and prolongs survival in NMC mouse xenograft models. Cayman's BRD4 bromodomains 1 and 2 (human, recombinant, aa 49-460) protein can be used for ELISA, Western blot (WB), and binding assay applications.Synonyms: BRD4, Bromodomain containing protein 4, HUNK1, MCAP. Purity: >70% estimated by SDS-PAGE. Source: Recombinant human GST-tagged protein expressed in E. coli. MW: 73.4 kDa. Formulation: (Request formulation change), 50 mM Tris, pH 7.5, with 500 mM sodium chloride, 5% glycerol, and 5 mM beta-mercaptoethanol. UniProt Accession #: O60885.