BAG family molecular chaperone regulator 3 (Bag3), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Yeast. Expression Region: 2-577aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: SAATQSPMMQ MASGNGASDR DPLPPGWEIK IDPQTGWPFF VDHNSRTTTW NDPRVPPEGP KDTASSANGP SRDGSRLLPI REGHPIYPQL RPGYIPIPVL HEGSENRQPH LFHAYSQPGV QRFRTEAAAA TPQRSQSPLR GGMTEAAQTD KQCGQMPATA TTAAAQPPTA HGPERSQSPA ASDCSSSSSS ASLPSSGRSS LGSHQLPRGY IPIPVIHEQN ITRPAAQPSF HQAQKTHYPA QQGEYQPQQP VYHKIQGDDW EPRPLRAASP FRSPVRGASS REGSPARSGT PVHCPSPIRV HTVVDRPQPM THREPPPVTQ PENKPESKPG PAGPDLPPGH IPIQVIRREA DSKPVSQKSP PPAEKVEVKV SSAPIPCPSP SPAPSAVPSP PKNVAAEQKA APSPAPAEPA APKSGEAETP PKHPGVLKVE AILEKVQGLE QAVDSFEGKK TDKKYLMIEE YLTKELLALD SVDPEGRADV RQARRDGVRK VQTILEKLEQ KAIDVPGQVQ VYELQPSNLE AEQPLQEIMG AVVADKDKKG PENKDPQTES QQLEAKAATP PNPSNPADSA GNLVAP. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity. Reference: Bis, a Bcl-2-binding protein that synergizes with Bcl-2 in preventing cell death.Lee J.H., Takahashi T., Yasuhara N., Inazawa J., Kamada S., Tsujimoto Y.Oncogene 18:6183-6190(1999). Function: Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70. Has anti-apoptotic activity. Plays a role in the HSF1 nucleocytoplasmic transport.