Atrial natriuretic peptide receptor 1 (Npr1), partial, recombinant mouse

Organism: Mus musculus (Mouse). Source: Mammalian cell. Expression Region: 29-469aa. Protein Length: Partial. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: SDLTVAVVLP LTNTSYPWSW ARVGPAVELA LGRVKARPDL LPGWTVRMVL GSSENAAGVC SDTAAPLAAV DLKWEHSPAV FLGPGCVYSA APVGRFTAHW RVPLLTAGAP ALGIGVKDEY ALTTRTGPSH VKLGDFVTAL HRRLGWEHQA LVLYADRLGD DRPCFFIVEG LYMRVRERLN ITVNHQEFVE GDPDHYTKLL RTVQRKGRVI YICSSPDAFR NLMLLALDAG LTGEDYVFFH LDVFGQSLQG AQGPVPRKPW ERDDGQDRRA RQAFQAAKII TYKEPDNPEY LEFLKQLKLL ADKKFNFTME DGLKNIIPAS FHDGLLLYVQ AVTETLAQGG TVTDGENITQ RMWNRSFQGV TGYLKIDRNG DRDTDFSLWD MDPETGAFRV VLNFNGTSQE LMAVSEHRLY WPLGYPPPDI PKCGFDNEDP ACNQDHFSTL E. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: n/a. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered PBS, 6% Trehalose, pH 7.4. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand. Reference: Retinal degeneration protein 3 controls membrane guanylate cyclase activities in brain tissue. Chen Y., Brauer A.U., Koch K.W. Front Mol Neurosci 15:1076430-1076430 (2022). Function: nan