Apoptosis-inducing factor 1, mitochondrial (AIFM1),partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 103-612aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: GLTPEQKQKK AALSASEGEE VPQDKAPSHV PFLLIGGGTA AFAAARSIRA RDPGARVLIV SEDPELPYMR PPLSKELWFS DDPNVTKTLR FKQWNGKERS IYFQPPSFYV SAQDLPHIEN GGVAVLTGKK VVQLDVRDNM VKLNDGSQIT YEKCLIATGG TPRSLSAIDR AGAEVKSRTT LFRKIGDFRS LEKISREVKS ITIIGGGFLG SELACALGRK ARALGTEVIQ LFPEKGNMGK ILPEYLSNWT MEKVRREGVK VMPNAIVQSV GVSSGKLLIK LKDGRKVETD HIVAAVGLEP NVELAKTGGL EIDSDFGGFR VNAELQARSN IWVAGDAACF YDIKLGRRRV EHHDHAVVSG RLAGENMTGA AKPYWHQSMF WSDLGPDVGY EAIGLVDSSL PTVGVFAKAT AQDNPKSATE QSGTGIRSES ETESEASEIT IPPSTPAVPQ APVQGEDYGK GVIFYLRDKV VVGIVLWNIF NRMPIARKII KDGEQHEDLN EVAKLFNIHE. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermbrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. Reference: Molecular characterization of mitochondrial apoptosis-inducing factor.Susin S.A., Lorenzo H.K., Zamzami N., Marzo I., Snow B.E., Brothers G.M., Mangion J., Jacotot E., Costantini P., Loeffler M., Larochette N., Goodlett D.R., Aebersold R., Siderovski D.P., Penninger J.M., Kroemer G.Nature 397:441-446(1999). Function: Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.