Aldehyde dehydrogenase family 1 member A3 (ALDH1A3), human, recombinant

Organism: Homo sapiens (Human). Source: Yeast. Expression Region: 2-512aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: ATANGAVENG QPDRKPPALP RPIRNLEVKF TKIFINNEWH ESKSGKKFAT CNPSTREQIC EVEEGDKPDV DKAVEAAQVA FQRGSPWRRL DALSRGRLLH QLADLVERDR ATLAALETMD TGKPFLHAFF IDLEGCIRTL RYFAGWADKI QGKTIPTDDN VVCFTRHEPI GVCGAITPWN FPLLMLVWKL APALCCGNTM VLKPAEQTPL TALYLGSLIK EAGFPPGVVN IVPGFGPTVG AAISSHPQIN KIAFTGSTEV GKLVKEAASR SNLKRVTLEL GGKNPCIVCA DADLDLAVEC AHQGVFFNQG QCCTAASRVF VEEQVYSEFV RRSVEYAKKR PVGDPFDVKT EQGPQIDQKQ FDKILELIES GKKEGAKLEC GGSAMEDKGL FIKPTVFSEV TDNMRIAKEE IFGPVQPILK FKSIEEVIKR ANSTDYGLTA AVFTKNLDKA LKLASALESG TVWINCYNAL YAQAPFGGFK MSGNGRELGE YALAEYTEVK TVTIKLGDKN P. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Seems to be the key enzyme in the formation of an RA gradient along the dorso-ventral axis during the early eye development and also in the development of the olfactory system. Reference: "Molecular cloning, genomic organization, and chromosomal localization of an additional human aldehyde dehydrogenase gene, ALDH6."Hsu L.C., Chang W.-C., Hiraoka L., Hsieh C.-L.Genomics 24:333-341(1994). Function: NAD-dependent aldehyde dehydrogenase that catalyzes the formation of retinoic acid