5'-Nucleotidase, Recombinant, Human (CD73, 5'-nucleotidase, ecto)

CD73, an ecto-5'-Nucleotidase, is an ectoenzyme expressed by most cell types. The 5'-Nucleotidase activity of CD73 converts extracellular nucleoside-5'-monophosphates to nucleosides, with AMP as the preferred substrate. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. These functions include the adhesion of B-cells to follicular dendritic cells and T-cell signaling CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'-Nucleotidase activity. The native enzyme is a homodimer bound to the cell membrane through a glycosyl phophatidylinositol (GPI) anchor. This recombinant human CD73 is truncated at the C-terminus, and lacks the GPI modification site. The recombinant CD73 is therefore secreted as a monomer. Recombinant protein corresponding to Trp27-Lys547, with a C-terminal 6-His tag from human 5'-Nucleotidase expressed in CHO cells (AAH65937). , SDS-PAGE: , 61-62kD under reducing conditions, Biological Activity: Measured by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-monophosphate. The specific activity is >15,000pmoles/min/ug. Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.