RPE-iFluor(TM) 750 Tandem

Ex: 565 nm. Em: 779 nm. Soluble in: Water. R-Phycoerythrin (R-PE) is isolated from red algae.  Its primary absorption peak is at 565 nm with secondary peaks at 496 and 545 nm.  The relative prominence of the secondary peaks varies significantly among R-PEs from different species.  R-PE has three types of subunits: _ (~20,000 daltons), _ (~20,000 daltons) and _ (~30,000 daltons). The molecular weight of intact R-PE has been found to be about 240,000 daltons, and a subunit structure of (__)6 _ has been determined. The _ subunit of R-PE contains only the phycoerythrobilin (PEB) chromophore, while _ and _ subunits contain both PEB and phycourobilin (PUB). Variability in the absorption spectra of R-PEs from various species reflects differences in the PEB/PUB ratio of the subunits. R-PE and closely related B-PE are the most intensely fluorescent phycobiliproteins, with quantum efficiencies probably in excess of 90%, and its orange fluorescence is readily visible by eye in any moderately concentrated solution. Our RPE-iFluorT 750 Tandem is an excellent replacement for RPE-Cy7® and RPE-Alexa Fluor® 750 Tandem due to their similar spectral properties. In general, our RPE Tandems have less residual RPE fluorescence, and are more photostable (in some cases) than RPE-Cy7® and RPE-Alexa Fluor® 750 Tandems.