Bromodeoxyuridine (BrdU) (5-Bromo-2'-deoxyuridine)

5-Bromo-2-deoxyuridine (BrdU) is an analog of thymidine, and can be incorporated into the newly synthesized DNA of replicating cells (S-phase cells). BrdU is a commonly used in the detection of proliferating cells in living tissues. It works by substituting for thymidine during DNA replication and incorporating itself into the newly synthesized DNA (during S-phase). Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Because BrdU can replace thymidine during the S phase of cell division, it can cause mutations. Bromodeoxyuridine (BrdU) is also used to prepare dNTP-BrdU solution for UV cross-linking of proteins to nucleic acids and to prepare complete MEM-10-Brdu or MEM-20-Brdu for vaccinia virus stock cultures. Synonyms: 5-Bromo-2'-deoxyuridine, 5-BrdU, 2'-Deoxy-5-bromorouridine, (+)-5-Bromo-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidine-2,4-dione, Bromodeoxyuridine, Broxuridine, NSC 38297, CAS Number: 59-14-3, Molecular Formula: C9H11BrN2O5, Molecular Weight: 307.10, Appearance: White to off white, crystalline or powder, Purity (HPLC): >98%, UV Content (on dry basis): >97%, Ratio of Absorbances (pH2): 0.47-0.53 (A250/A260), 1.87-1.93 (A280/A260), 1.45-1.55 (A290/A260), Melting Point: ~187-189°C, Specific Rotation (C=1%, H2O): +23.0°, Water (KF): <1%, Solubility: Soluble in 0.1N NaOH and DMF, sparingly soluble in water, slightly soluble in ethanol and acetone, practically insoluble in ether, RNase: None Detected, DNase: None Detected, Protease: None Detected, Endotoxin: <0.5EU/mg, Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards:  All products should be handled by qualified personnel only, trained in laboratory procedures.