Tetramethylrhodamine ethyl ester (perchlorate)

Tetramethylrhodamine ethyl ester (TMRE) (perchlorate) is a non-cytotoxic cell-permeant fluorogenic dye most commonly used to assess mitochondrial function using live cell fluorescence microscopy and flow cytometry. It displays excitation/emission spectra of 550/575 nm, respectively. Due to the polarization of the mitochondrial membrane, TMRE is taken up into healthy mitochondria. However, when the membrane is depolarized, as in apoptosis, it is not taken up or is released from the mitochondria. Thus, the strength of the fluorescence signal in mitochondria is used to assess cell viability.Formal Name: 3,6-bis(dimethylamino)-9-[2-(ethoxycarbonyl)phenyl]-xanthylium, perchlorate. CAS Number: 115532-52-0. Synonyms: TMRE, TMR ethyl ester. Molecular Formula: C26H27N2O3 . ClO4. Formula Weight: 515.0. Purity: >98%. Emission: 575 nm. Excitation: 550 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMSO: 30 mg/ml, Ethanol: slightly soluble, PBS (pH 7.2): slightly soluble. lambdamax: 226, 256, 352, 399, 550 nm. SMILES: O=C(OCC)C(C=CC=C1)=C1C2=C3C=CC(N(C)C)=CC3=[O+]C4=C2C=CC(N(C)C)=C4.O=Cl(=O)([O-])=O. InChi Code: InChI=1S/C26H27N2O3.ClHO4/c1-6-30-26(29)20-10-8-7-9-19(20)25-21-13-11-17(27(2)3)15-23(21)31-24-16-18(28(4)5)12-14-22(24)25,2-1(3,4)5/h7-16H,6H2,1-5H3,(H,2,3,4,5)/q+1,/p-1. InChi Key: NBAOBNBFGNQAEJ-UHFFFAOYSA-M.