SC-13

SC-13 is an inhibitor of flap endonuclease 1 (FEN1, IC50 = 4.2 nM) and an activator of stimulator of interferon genes (STING). It increases the levels of gamma histone H2AX (gammaH2AX) in MCF-7 and MDA-MB-231 breast cancer cells when used at a concentration of 40 µM. SC13 (20 or 50 µM) induces chromosomal aberrations in MCF-7 cells. It potentiates the proliferation inhibition induced by cisplatin (Cay-13119), 5-fluorouracil (Cay-14416), or temozolomide (Cay-14163) in MCF-7 cells when used at a concentration of 20 µM. SC-13 (20 µM) increases levels of cyclic GMP-AMP (cGAMP) synthase (cGAS), phosphorylated STING, chemokine (C-C motif) ligand 5 (CCL5), and CCL10 in HeLa cervical cancer and MCF-7 cells. In vivo, SC-13 (5 mg/kg every other day), in combination with T cells expressing chimeric antigen receptors (CARs), reduces tumor volume and weight without affecting body weight, as well as increases the number of CD8+ T cell tumor infiltrates, in a HeLa mouse xenograft model.Formal Name: N-[3-[(3,4-dihydro-2,4-dioxo-3-propylthieno[3,2-d]pyrimidin-1(2H)-yl)methyl]phenyl]-benzeneacetamide. CAS Number: 2098776-03-3. Synonyms: FEN1 inhibitor SC-13, FEN1-IN-SC-13, Flap Endonuclease 1 Inhibitor SC-13, Flap Endonuclease 1-IN-SC-13. Molecular Formula: C24H23N3O3S. Formula Weight: 433.5. Purity: >98%. Formulation: (Request formulation change), A solid. Solubility: Acetonitrile: Soluble, DMSO: Soluble. SMILES: O=C(NC1=CC=CC(CN(C2=C3SC=C2)C(N(CCC)C3=O)=O)=C1)CC4=CC=CC=C4. InChi Code: InChI=1S/C24H23N3O3S/c1-2-12-26-23(29)22-20(11-13-31-22)27(24(26)30)16-18-9-6-10-19(14-18)25-21(28)15-17-7-4-3-5-8-17/h3-11,13-14H,2,12,15-16H2,1H3,(H,25,28). InChi Key: USTMMKOHIRMBIV-UHFFFAOYSA-N.