N-Succinimidyl-S-acetylthioacetate

N-Succinimidyl-S-acetylthioacetate is a protein-modifying agent. It introduces sulfhydryl groups to proteins via S-acetylthioacetylation of free amine groups, which can be subsequently deprotected by deacetylation to reveal an unprotected thiol. N-Succinimidyl-S-acetylthioacetate has been used in conjunction with the protein cross-linking reagent SMCC (Cay-28955) in the conjugation of proteins with IgG antibodies for use in ELISAs.Formal Name: 2-(acetylthio)-acetic acid, 2,5-dioxo-1-pyrrolidinyl ester. CAS Number: 76931-93-6. Synonyms: SATA. Molecular Formula: C8H9NO5S. Formula Weight: 231.2. Purity: >90%. Formulation: (Request formulation change), A solid. Solubility: DMF: 16 mg/ml, DMSO: 16 mg/ml, Ethanol: Slightly soluble. SMILES: O=C(CSC(C)=O)ON1C(CCC1=O)=O. InChi Code: InChI=1S/C8H9NO5S/c1-5(10)15-4-8(13)14-9-6(11)2-3-7(9)12/h2-4H2,1H3. InChi Key: FLCQLSRLQIPNLM-UHFFFAOYSA-N.