Mitoxantrone (hydrochloride)

Mitoxantrone is an inhibitor of DNA topoisomerase IIalpha and HIV-1 integrase (IC50s = 5.3 and 3.8 µM, respectively). It intercalates into DNA in a cell-free assay when used at a concentration of 2 µM, as well as inhibits DNA synthesis and induces DNA-protein crosslinks in vitro in a concentration-dependent manner. Mitoxantrone inhibits the growth of K562 leukemia and K562-derived etoposide-resistant K/VP.5 cells (IC50s = 0.42 and 1.68 µM, respectively). It also inhibits the membrane fusion of a mixed population of HEK293T cells expressing either angiotensin-converting enzyme 2 (ACE2) or the extracellular domain of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein, also known as surface glycoprotein. Mitoxantrone (0.2 µM) inhibits the entry of SARS-CoV-2 into Vero E6 cells. Formulations containing mitoxantrone have been used in the treatment of cancer and multiple sclerosis.Formal Name: 1,4-dihydroxy-5,8-bis[[2-[(2-hydroxyethyl)amino]ethyl]amino]-9,10-anthracenedione, dihydrochloride. CAS Number: 70476-82-3. Synonyms: NCI 301739, NSC 301739. Molecular Formula: C22H28N4O6 . 2HCl. Formula Weight: 517.4. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 50 mg/ml, DMSO: 50 mg/ml, Ethanol: 5 mg/ml, PBS (pH 7.2): 10 mg/ml. lambdamax: 222, 242, 276, 610, 662 nm. SMILES: O=C1C2=C(C(O)=CC=C2O)C(C3=C1C(NCCNCCO)=CC=C3NCCNCCO)=O.Cl.Cl. InChi Code: InChI=1S/C22H28N4O6.2ClH/c27-11-9-23-5-7-25-13-1-2-14(26-8-6-24-10-12-28)18-17(13)21(31)19-15(29)3-4-16(30)20(19)22(18)32,,/h1-4,23-30H,5-12H2,2*1H. InChi Key: ZAHQPTJLOCWVPG-UHFFFAOYSA-N.