IPTG Dioxane Free (Isopropyl-beta-D-thiogalactoside(galactopyranoside))

IPTG is a carbohydrate used to induce b-galactosidase for the selection of recombinant plasmids. Used to select for lac Y mutants and to induce the lac operon in E. coli. IPTG will also induce the cellular content of lactose permease. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in E. coli. Synonyms: IPTG, Isopropyl-ß-D-thiogalactopyranoside, Isopropyl-ß-D-thiogalactoside, CAS No: 367-93-1, Molecular Formula: C9H18O5S, Molecular Weight: 238.31, Appearance: White crystalline or powder, Purity: >99%, Identity (IR): Conforms to structure, pH (5%): 5.0-7.0, Solubility: Colorless, clear, complete, Dioxane: Not Detected, Specific Rotation (C=1,H2O): -29° to -35°, Melting Point: 110-114°C, Water (KF): <1.0%, Blue-White Assay: Induces b-galactosidase in a pUC 18-containing strain. Storage and Stability: Stable for 12 months after receipt at -20°C. For long-term storage of reconstituted product, aliquot and store at -20°C. Further dilutions can be made in assay buffer.