IPA-3

IPA-3 is an allosteric inhibitor of p21-activated kinase 1 (PAK1) activation (IC50 = 2.5 µM) that does not inhibit pre-activated PAK1. It selectively inhibits activation of the group I PAKs, PAK1, PAK2, and PAK3 over the group II PAKs, PAK4, PAK5, and PAK6 at 10 µM. IPA-3 inhibits the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) endoribonuclease nsp15 in a FRET assay (IC50 = 6.8 µM) and inhibits SARS-CoV-2 replication in infected Vero CCL-81 cells (IC50 = 8.5 µM). It inhibits the proliferation of H2M and MHCC97-L metastatic hepatocellular carcinoma (HCC) and non-metastatic HepG2 HCC cells when used at concentrations of 5 and 10 µM. IPA-3 (2 and 4 mg/kg) reduces tumor growth in an MHCC97-L cell mouse xenograft model.Formal Name: 1,1'-dithiobis-2-naphthalenol. CAS Number: 42521-82-4. Synonyms: Inhibitor of PAK1 Activation 3. Molecular Formula: C20H14O2S2. Formula Weight: 350.5. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 5 mg/ml, DMF:PBS (pH 7.2) (1:4): 0.2 mg/ml, DMSO: 5 mg/ml. lambdamax: 223, 357 nm. SMILES: OC1=C(SSC2=C(O)C=CC3=C2C=CC=C3)C4=CC=CC=C4C=C1. InChi Code: InChI=1S/C20H14O2S2/c21-17-11-9-13-5-1-3-7-15(13)19(17)23-24-20-16-8-4-2-6-14(16)10-12-18(20)22/h1-12,21-22H. InChi Key: RFAXLXKIAKIUDT-UHFFFAOYSA-N.