Ilomastat (GM6001, MMP Inhibitor),

GM6001 MMP Inhibitor (Ilomastat) is a potent inhibitor of collagenase. Broad spectrum inhibitor of matrix metalloproteases (MMPs) with Ki factor in nM range. Widely used to inhibit MMPs in vitro experiments and in animal models. Nomenclature: N-[(2R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl]-L-tryptophan methylamide , (2R)-N'-hydroxy-N-[(2S)-3-(5H-indol-3-yl)-1-methylamino-1-oxopropan-2-yl]-2-(2-methylpropyl)butanediamide, Reported Ki values are as follows (see Galardy Ref): Human MMP-1 (Fibroblast collagenase): 0.4nM, Human MMP-3 (Stromelysin): 27nM, Human MMP-2 (72kD gelatinase): 0.5nM, Human MMP-8 (Neutrophil collagenase):0.1nM, Human MMP-9 (92kD gelatinase): 0.2nM, Applications: Suitable for use in Cell Culture. Recommended Dilutions: Cell Culture: 10-25uM (1mg/ml in DMSO = 2.57mM) , Recommended Dilution Buffer: , 50mM Tris pH7.5, 150mM NaCl, 20mM CaCl2-2H2O. Recommended Dilution Concentration: It is recommended that the concentration of GM6001 in aqueous working solutions is determined upon dilution and confirmed prior to each use. Relative concentrations of GM6001 in aqueous solutions may be determined by measuring the absorbance of the solution at 280nm. Quality Control: H-NMR Spectrum: Conforms, Mass Spectrum: 389 [M+1], Thin Layer Chromatography (ethylacetate:methanol = 6:1): Single spot, Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.