GW2580, Free Base (5-[[3-methoxy-4-[(4-methoxyphenyl)methoxy]phenyl]methyl]pyrimidine-2,4-diamine)

GW2580 is a small-molecule inhibitor of cFMS kinase, at 60nM it inhibited human cFMS kinase completely in vitro and was inactive against 26 other kinases. GW2580 inhibited lipopolysaccharide (LPS)-induced TNF production in mice, but not LPS-induced TNF production in freshly isolated human monocytes and mouse macrophages in vitro. Also, GW2580 at 1uM totally inhibited CSF-1-induced growth of mouse M-NFS-60 myeloid cells and human monocytes, and prevented bone degradation in cultures of rat calvaria and rat fetal long bone in vitro. Melting Point: 209-210.5°C, Elemental Analysis: Calculated, C = 65.56%, H = 6.05%, N = 15.29%, Found C = ~65%, H = ~6%, N = ~15%, Solubility: Soluble in DMSO at 40mg/ml, very poorly soluble in ethanol, very poorly soluble in water, maximum solubility in plain water is estimated to be about 20-100uM, buffers, serum, or other additives may increase or decrease the aqueous solubility. Storage and Stability: Long-term storage is recommended at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.