CU-T12-9

CU-T12-9 is an agonist of the toll-like receptor 1/2 (TLR1/2) heterodimer. It is selective for TLR1/2 over the TLR2/6 heterodimer in a secreted alkaline phosphatase (SEAP) assay at concentrations up to 10 µg/ml using HEK-Blue cells expressing human TLR2 and endogenously expressing both TLR1 and TLR6 and anti-TLR antibodies. CU-T12-9 (5 µM) induces NF-kappaB signaling in a reporter assay. It also increases nitric oxide (NO) production in RAW 264.7 cells and primary rat macrophages at 3.6 and 0.4 µM, respectively, and TNF-alpha production in RAW 264.7 cells (EC50 = 0.06 µM).Formal Name: N-methyl-4-nitro-2-[4-[4-(trifluoromethyl)phenyl]-1H-imidazol-1-yl]-benzenamine. CAS Number: 1821387-73-8. Molecular Formula: C17H13F3N4O2. Formula Weight: 362.3. Purity: >98%. Formulation: (Request formulation change), A solid. Solubility: DMSO: 30 mg/ml. SMILES: CNC(C(N1C=C(C2=CC=C(C(F)(F)F)C=C2)N=C1)=C3)=CC=C3[N+]([O-])=O. InChi Code: InChI=1S/C17H13F3N4O2/c1-21-14-7-6-13(24(25)26)8-16(14)23-9-15(22-10-23)11-2-4-12(5-3-11)17(18,19)20/h2-10,21H,1H3. InChi Key: LITXVDAFEYLWQE-UHFFFAOYSA-N.