Caylin-1

Nutlin-3 is an activator of p53 that functions by inhibiting the interaction of p53 with MDM2, a negative regulator of p53 activity. Caylin-1 is a nutlin-3 analog which contains chlorine substituents at the 3 and 4 positions on two of the phenyl rings rather than a single 4-chloro as seen in nutlin-3. At high concentrations, caylin-1 inhibits the growth of HCT116 cells with an IC50 value of approximately 7 µM, making it about 7-fold less potent than nutlin-3 in the same assay. Interestingly, at concentrations at or below 1 µM, caylin-1 promotes the growth of HCT116 cells approximately 20% compared to untreated cells. The mechanism of the growth promoting properties of caylin-1 have not yet been elucidated.Formal Name: 4-[4,5-bis(3,4-chlorophenyl)-2-(2-isopropoxy-4-methoxy-phenyl)-4,5-dihydro-imidazole-1-carboxyl]-piperazin-2-one. CAS Number: 1207480-88-3. Molecular Formula: C30H28Cl4N4O4. Formula Weight: 650.4. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMSO: 30 mg/ml, Ethanol: 30 mg/ml, Ethanol:PBS (pH 7.2)(1:3): .25 mg/ml. SMILES: COc1ccc(c(OC(C)C)c1)C1=N[C@@H](c2ccc(Cl)c(Cl)c2)[C@@H](c2ccc(Cl)c(Cl)c2)N1C(=O)N1CCNC(=O)C1. InChi Code: InChI=1S/C30H28Cl4N4O4/c1-16(2)42-25-14-19(41-3)6-7-20(25)29-36-27(17-4-8-21(31)23(33)12-17)28(18-5-9-22(32)24(34)13-18)38(29)30(40)37-11-10-35-26(39)15-37/h4-9,12-14,16,27-28H,10-11,15H2,1-3H3,(H,35,39)/t27-,28+/m0/s1. InChi Key: SOQGENPVVRSGAY-WUFINQPMSA-N.