Biotin-NHS

N-Hydroxysuccinimide (NHS) activates carboxylic acid groups (on biotin) to facilitate coupling reactions. Biotin-NHS is a compound used to attach biotin to primary amines under alkaline conditions (pH~8-9). For example, lysines on the surface of proteins, including antibodies, are ideal targets for biotinylation with this compound.Formal Name: 6aR-hexahydro-2-oxo-2,5-dioxo-1-pyrrolidinyl ester-1H-thieno[3aS,4-d]imidazole-4S-pentanoic acid. CAS Number: 35013-72-0. Synonyms: Biotin N-hydroxysuccinimide ester, NSC 345668. Molecular Formula: C14H19N3O5S. Formula Weight: 341.4. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 20 mg/ml, DMSO: 20 mg/ml, DMSO:PBS (pH 7.2) (1:1): 0.5 mg/ml. SMILES: O=C1N[C@H]2CS[C@@H](CCCCC(=O)ON3C(=O)CCC3=O)[C@H]2N1. InChi Code: InChI=1S/C14H19N3O5S/c18-10-5-6-11(19)17(10)22-12(20)4-2-1-3-9-13-8(7-23-9)15-14(21)16-13/h8-9,13H,1-7H2,(H2,15,16,21)/t8-,9-,13-/m0/s1. InChi Key: YMXHPSHLTSZXKH-RVBZMBCESA-N.