8-pCPT-2'-O-methyl Cyclic AMP

Exchange proteins activated by cAMP (Epacs) are guanine nucleotide exchange factors (GEFs) for the small GTPases Rap1 and Rap2. 8-pCPT-2'-O-methyl Cyclic AMP (8-pCPT-2'-O-Me-cAMP) is an 8-(4-chlorophenylthio) analog of cAMP that activates Epacs (AC50 = 1.8 µM). It is a super-activator of Epacs in that it dissociates GDP from Rap1 more strongly than the natural Epac agonist, cAMP. 8-pCPT-2'-O-Me-cAMP is strongly selective for Epac over the cAMP-activated kinase PKA. It does not discriminate between Epac1 and Epac2 and is used extensively to elucidate the roles of these Rap GEFs in cell function.Formal Name: 8-[(4-chlorophenyl)thio]-2'-O-methyl-adenosine cyclic 3',5'-(hydrogen phosphate), monosodium salt. CAS Number: 634207-53-7. Synonyms: 8-CPT-2-methyl cAMP, 8-CPT-2-methyl Cyclic adenosine monophosphate, 8-pCPT cAMP, 8-pCPT Cyclic adenosine monophosphate, 8-pCPT Cyclic AMP, 8-pCPT-2'-O-methyl cAMP, 8-pCPT-2'-O-methyl Cyclic adenosine monophosphate. Molecular Formula: C17H16N5O6ClPS . Na. Formula Weight: 507.8. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMSO: 25 mg/ml, Ethanol: 0.5 mg/ml, PBS (pH 7.2): 10 mg/ml. lambdamax: 210, 224, 283 nm. SMILES: O=P1([O-])O[C@H]2[C@@H](OC)[C@H](N3C(SC4=CC=C(Cl)C=C4)=NC5=C3N=CN=C5N)O[C@@H]2CO1.[Na+]. InChi Code: InChI=1S/C17H17ClN5O6PS.Na/c1-26-13-12-10(6-27-30(24,25)29-12)28-16(13)23-15-11(14(19)20-7-21-15)22-17(23)31-9-4-2-8(18)3-5-9,/h2-5,7,10,12-13,16H,6H2,1H3,(H,24,25)(H2,19,20,21),/q,+1/p-1/t10-,12-,13-,16-,/m1./s1. InChi Key: VHTMKTNUXDACHB-KHXPSBENSA-M. License: Protected by Patent No. EP 02077219.0 and foreign equivalents. Exclusively licensed to BIOLOG Life Science Institute for research purposes only..