7-methyl-6-Thioguanosine (technical grade)

7-methyl-6-Thioguanosine is a chromophoric substrate that is used to quantify inorganic phosphate (Pi). In the presence of purine nucleoside phosphorylase, it reacts with Pi to produce 7-methyl-6-thioguanine and ribose 1-phosphate, with the increase in 7-methyl-6-thioguanine followed by continuous spectrophotometry at an absorbance of 355-360 nm. 7-methyl-6-Thioguanosine is also used to measure changes in Pi levels in coupled enzyme systems, including assays of the activities of protein phosphatases, phosphorylase kinases, GTPases, and ATPases.Formal Name: 2-amino-6,9-dihydro-7-methyl-9-beta-D-ribofuranosyl-6-thioxo-1H-purinium, inner salt. CAS Number: 55727-10-1. Synonyms: MESG. Molecular Formula: C11H15N5O4S. Formula Weight: 313.3. Purity: >85%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 50 mg/ml, DMSO: 30 mg/ml, PBS (pH 7.2): 10 mg/ml. lambdamax: 212, 263, 345 nm. SMILES: O[C@H]1[C@@H](O)[C@H](N2C=[N+](C)C3=C2N=C([NH-])NC3=S)O[C@@H]1CO. InChi Code: InChI=1S/C11H15N5O4S/c1-15-3-16(8-5(15)9(21)14-11(12)13-8)10-7(19)6(18)4(2-17)20-10/h3-4,6-7,10,17-19H,2H2,1H3,(H2-,12,13,14,21)/t4-,6-,7-,10-/m1/s1. InChi Key: RFHIWBUKNJIBSE-KQYNXXCUSA-N.