5(6)-FAM N-succinimidyl ester

5(6)-FAM N-succinimidyl ester
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
CDX-C0011-M100 100 mg - -

3 - 9 Werktage*

128,00 €
 
Yellow to orange powder. Soluble in DMSO or dimethylformamide. Fluorescent probe and cell... mehr
Produktinformationen "5(6)-FAM N-succinimidyl ester"
Yellow to orange powder. Soluble in DMSO or dimethylformamide. Fluorescent probe and cell staining dye. Reacts with primary and secondary amine groups on proteins and other biomolecules. Cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules. Due to this covalent coupling reaction fluorescent CFSE can be retained within cells for extremely long periods. Used for immunological studies to monitor the proliferation of many cell types (including lymphocytes, fibroblasts) and even bacteria. Has been used to prepare fluorescent derivatives of tubulin and for the preparation of a phenytoin tracer for the determination of this drug in serum and plasma by fluorescence polarization immunoassay (FPIA). Favored over FITC in bioconjugations, since FAM reagents are more resistant to hydrolysis. In addition, FAM reagents require less stringent conjugation conditions and give better conjugation yields, and the resulted conjugates have superior stability. Spectral properties: lambdaex= 492, lambdaem=517nm in DMF or 0.1 M Tris, pH 8.0.
Schlagworte: 5(6)-Carboxyfluorescein NHS ester (mixture of isomers), 5(6)-CFSE, 5(6)-FAM SE, FLUOS
Hersteller: Chemodex
Hersteller-Nr: C0011

Eigenschaften

Anwendung: Fluorescent probe, Cell staining probe, Amine reactive
MW: 473,4 D
Formel: C25H15NO9
Reinheit: >98%
Format: Solid

Datenbank Information

CAS : 117548-22-8| Finde Alternativen

Handhabung & Sicherheit

Lagerung: -20°C
Versand: +20°C (International: +20°C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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