1-NM-PP1

1-NM-PP1 is a cell permeable inhibitor of kinases that have been mutated, by a single base substitution, to become 'analog sensitive' (as), as compared to the wild-type kinase. 1-NM-PP1 was first developed to optimally inhibit v-Src-as1, with an I338G substitution, preferentially over v-Src (IC50 = 4.2 nM versus 28 µM, respectively). The homologous mutation in other kinases generated similar analog sensitivity (e.g., IC50 = 3.2 nM for c-Fyn-as1 versus 1.0 µM for c-Fyn, 5.0 nM for Cdk2-as1 versus 29 µM for Cdk2, 8.0 nM for CAMKII-as1 versus 24 µM for CAMKII). This approach has been used to elucidate functions of several kinases in both mammalian and yeast systems.Formal Name: 1-(1,1-dimethylethyl)-3-(1-naphthalenylmethyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine. CAS Number: 221244-14-0. Synonyms: PP1 Analog II. Molecular Formula: C20H21N5. Formula Weight: 331.4. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMF:PBS (pH 7.2) (1:5): 0.15 mg/ml, DMSO: 20 mg/ml, Ethanol: 2 mg/ml. lambdamax: 224, 282 nm. SMILES: NC1=NC=NC2=C1C(CC3=CC=CC4=C3C=CC=C4)=NN2C(C)(C)C. InChi Code: InChI=1S/C20H21N5/c1-20(2,3)25-19-17(18(21)22-12-23-19)16(24-25)11-14-9-6-8-13-7-4-5-10-15(13)14/h4-10,12H,11H2,1-3H3,(H2,21,22,23). InChi Key: GDQXJQSQYMMKRA-UHFFFAOYSA-N.