1-Methylpseudouridine

1-Methylpseudouridine is a nucleoside analog that has been found in Streptomyces platensis. It increases the thermodynamic and biological stability of RNA. Unlike unmodified mRNA, 1-methylpseudouridine-containing mRNA does not induce IFN-beta secretion in A549 cells, indicating low immunogenicity. Intramuscular or intradermal administration of luciferase mRNA containing 1-methylpseudouridine via lipid complexes increases luciferase activity in mice. Formulations containing 1-methylpseudouridine incorporated into mRNA have been used in the development of mRNA-based vaccines.Formal Name: 1-methyl-5-beta-D-ribofuranosyl-2,4(1H,3H)-pyrimidinedione. CAS Number: 13860-38-3. Synonyms: m1psi, N1-methyl-psi, N1-Methylpseudouridine, NSC 240023, U-50228. Molecular Formula: C10H14N2O6. Formula Weight: 258.2. Purity: >98%. Formulation: (Request formulation change), A solid. Solubility: DMF: 3 mg/ml, DMSO: 10 mg/ml, PBS (pH 7.2): 5 mg/ml. lambdamax: 211, 271 nm. SMILES: O[C@H]1[C@@H](O)[C@@](C2=CN(C)C(NC2=O)=O)([H])O[C@@H]1CO. InChi Code: InChI=1S/C10H14N2O6/c1-12-2-4(9(16)11-10(12)17)8-7(15)6(14)5(3-13)18-8/h2,5-8,13-15H,3H2,1H3,(H,11,16,17)/t5-,6-,7-,8+/m1/s1. InChi Key: UVBYMVOUBXYSFV-XUTVFYLZSA-N. Origin: Synthetic.